Hydrophilic Interaction Liquid Chromatography–Tandem Mass Spectrometry of 5-Aminolevulinic Acid for Low Concentration and Small Sample Volumes

Plasma Free Metanephrines Quantitation with Automated Online Sample Preparation and Liquid ChromatographyTandem Mass Spectrometry

Recently, we reported an LC-MS/MS method for measuring Pmets using ion-pairing solid phase extraction (IP-SPE) and porous graphitic carbon (PGC) column chromatography1, 2. Although the method is fast and analytically sensitive, it can be further improved by automating the offline sample preparation with online sample preparation technology, which is more timeand cost-effective. Thermo Scientifi...

Determination of 3'-phosphoadenosine-5'- phosphosulfate in cells using hydrophilic interaction liquid chromatography and mass spectrometry

Hydrophilic Interaction Liquid Chromatography Coupled to 1 Mass Spectrometry for the Characterization of Prebiotic

Hydrophilic interaction liquid chromatography-mass spectrometry of (lyso)phosphatidic acids, (lyso)phosphatidylserines and other lipid classes.

The goal of this work is a systematic optimization of hydrophilic interaction liquid chromatography (HILIC) separation of acidic lipid classes (namely phosphatidic acids-PA, lysophosphatidic acids-LPA, phosphatidylserines-PS and lysophosphatidylserines-LPS) and other lipid classes under mass spectrometry (MS) compatible conditions. The main parameters included in this optimization are the type ...

Determination of Oxidized Phosphatidylcholines by Hydrophilic Interaction Liquid Chromatography Coupled to Fourier Transform Mass Spectrometry

A novel liquid chromatography-mass spectrometry (LC-MS) approach for analysis of oxidized phosphatidylcholines by an Orbitrap Fourier Transform mass spectrometer in positive electrospray ionization (ESI) coupled to hydrophilic interaction liquid chromatography (HILIC) was developed. This method depends on three selectivity criteria for separation and identification: retention time, exact mass a...